abstract
recent studies into the beneficial effects of fermented foods have shown that this class of foods are effective in managing hyperuricemia and gout. in this study, the uric acid (ua) degradation ability oflimosilactobacillus fermentumjl-3 strain, isolated from “jiangshui” (a fermented chinese food), was investigated.in vitroresults showed that jl-3 strain exhibited high degradation capacity and selectivity toward ua. after oral administration to mice for 15days, jl-3 colonization was continuously detected in the feces of mice. the ua level in urine of mice fed with jl-3 was similar with the control group mice. and the serum ua level of the former was significantly lower (31.3%) than in the control, further confirmed the ua-lowering effect of jl-3 strain.limosilactobacillus fermentumjl-3 strain also restored some of the inflammatory markers and oxidative stress indicators (il-1β, mda, cre, blood urea nitrogen) related to hyperuricemia, while the gut microbial diversity results showed that jl-3 could regulate gut microbiota dysbiosis caused by hyperuricemia. therefore, the probioticlimosilactobacillus fermentumjl-3 strain is effective in lowering ua levels in mice and could be used as a therapeutic adjunct agent in treating hyperuricemia.
keywords:
1. introduction
hyperuricemia is one of the major metabolic diseases associated with gout which is on a rise in many countries.some studies have reported that the prevalence of gout is from 1% to 3% in europe and 3.9% in the us.,also, more than 7.44 million cases of gout were estimated to have occurred worldwide in 2017.according to the gout report white paper (2017), hyperuricemia patients in china had reached 170 million, of which approximately 47% developed gout with an annual growth rate of 9.7%.foods rich in the purines are incompletely degraded to ua in humans, resulting in high levels of ua in the plasma.,hence, ua accumulation in tissues as urate, which had been considered as an important risk factor for the development of gout and other diseases such as cardiovascular disease, endothelial dysfunction and metabolic syndrome.,
high levels of ua in plasma have long been associated with hyperuricemia.there are two ways of ua accumulation in humans. first is an endogenous buildup caused by enzyme deficiencies in the purine metabolism pathway. this deficiency in major purine metabolism enzymes leads to an increased rate of nucleic acid decomposition and ua production, which is under the control of a mechanism whereby an end product control itself by inhibiting the enzyme producing it.another route of ua accumulation is through the intake and exogenous absorption of purine rich foods. a recent study showed that the intake of meat increases the risk of gout by 21%, while sea food intake increases its risk by just 7%.hyperuricemia can be alleviated effectively by external interventions.in general, it can also be managed through the excretion of ua in large amounts by the kidneys, while only 30% of the total ua is degraded by the action of intestinal flora burden per time.,,however, the effectiveness of the intestinal flora can be greatly influenced by purine-rich foods, which may disturb the purine metabolism process, thereby elevating serum ua levels.,it is thus necessary to control ua from external sources to prevent hyperuricemia.
currently, several methods are used to treat hyperuricemia, including diet, drugs and biotherapy, aimed at purine uptake and degradation.,,an example is the representative ua-lowering drug, allopurinol, which can competitively bind with the xanthine oxidase (xod) enzyme to reduce ua production.febuxostat is another novel non-purine selective inhibitor of xod.both drugs are widely used to treat hyperuricemia and they are relative safety.dietary interventions work by restricting the intake of high-purine foods and alcohol, but can be less effective than drugs because of the difficulty in adhering to dietary restrictions for a long time. microbial remediation as a cost-effective treatment approach has been greatly accepted by the public due to its minimal site disruptions in the body.thus, severallactobacillusstrains that can utilize purines have been discovered. among these are the strains found to be effective in lowering ua in rodents.l. brevis(dm9218) andl. gasseri(pa-3) for instance degraded the intermediate products of purine metabolism to ameliorate hyperuricemia.,for literature, most of theselactobacillusstrains have also been aimed at intestinal degradation and absorption of purines.
fermented foods are rich in lactic acid bacteria, and can produce organic acids to control decaying microorganisms and pathogens.,research has shown that fermented foods such as yogurt are beneficial for type 2 diabetes patients, because of its richness in lactic acid bacteria.probiotics have also been reported to be effective in degrading ua, so we hypothesized that the activity of human intestinal flora can be enhanced by probiotics.,“jiangshui” is a traditional fermented food in northwestern china, which is made with vegetables, such as celery and cabbage. lactic acid bacteria, acetic acid bacteria and yeast are the main microbes in the jiangshui fermentation process, especially the facultative anaerobic lactic acid bacteria.thus, the present study is aimed at screening microbial strains from fermented jiangshui with ua degradation ability to evaluate the effects of these strains on ua-induced hyperuricemia in a mouse model.
2. result:
2.1. correlation between the frequency of jiangshui noodle and gout
to explore the correlation between the consumption of jiangshui and the incidence of gout, a questionnaire survey was conducted on 180 residents in lanzhou city. the results showed that when the consumption frequency of jiangshui noodle exceeded six times a week, the prevalence of gout displayed a downtrend (supplementary file 2,). also, pearson’s analysis indicated a negative correlation between jiangshui noodle and gout (supplementary file 2,), which suggested that people who regularly ate jiangshui noodles were less likely to develop gout.
figure 1.screening and identification of l-3 strain in jiangshui. a]scanning electron microscope morphology of jl-3.b]phylogenetic tree of strain jl-3 and related bacteria.c]the effects of different media on the growth of jl-2, jl-3, and jl-8. compared with jl-2 and jl-8, jl-3 had the best growth effect in the medium containing ua, and there was no growth phenomenon in the medium without ua.d]identification of jl-3 in 10 kinds of jiangshui bought from lanzhou supermarket (gansu, china)
2.2. isolation of ua degrading strains and product identification
to explore whether probiotics in jiangshui had beneficial effects on gout, twenty strains with ua resistance activity were isolated from commercially fermented jiangshui and cultured in a medium containing ua as the sole carbon and nitrogen source. three strains jl-2, jl-3 and jl-8 could survive, of which the growth rate of jl-3 was significantly higher than the other two strains, showing an od600value above 0.25 within a 24h of incubation period (). when compared with the ncbi blast database, the jl-3 strain was closely related tolimosilactobacillus fermentum(). transmission election microscopy (tem) also showed that jl-3 had a typical morphology oflimosilactobacillus bacilli().
to analyze the ua degrading ability of jl-3, it was added to a sterile phosphate-buffered saline (pbs) solution containing ua (10mmol/l) for cultivation. results showed that the jl-3 strain could degrade 40.9% of ua within 24h, compared to xs-14 which is alimosilactobacillus fermentumstrain from yogurt (). alternatively, high power liquid chromatography (hplc) results revealed absorption peaks of ua and that of its degradation products at 4.868, 2.255 and 1.976 respectively, which was consistent with the standard of ua, allantoin and urea peaks (). this suggested that jl-3 could break down ua into smaller molecules such as allantoin and urea. simultaneously, polymerase chain reaction (pcr) results of different commercial jiangshui showed that jl-3 is widely present in various jiangshui samples ().
figure 2.detection of ua degradation products by jl-3. a]the ua degradation ability of the jl-3 strainin-vitro.b]the ua degradation products of jl-3 detected by hplc. a. detection peak of ua standard; b. detection peak of allantoin standard; c. detection peak of urea standard; d. the main metabolism products of jl-3 were urea and allantoin. bars showed the mean ± sd (n=5 mice per group) **p<.01; ****p<.0001
2.3. jl-3 decreases ua level in hyperuricemic mice
the previous experiment proved that jl-3 could degrade ua. to test its ability in mice, the hyperuricemia mice model was constructed (). the constructed model of the hyperuricemia mice efficiently elevated serum ua in mice more stably, compared with oteracil potassium or high purine diet alone to sustain a longer duration of hyperuricemia.,,thus, this method was adopted to construct hyperuricemia mice and gavaged with the strain (). feces of mice were collected to investigate the survival of jl-3. the relative abundance of jl-3 strain in the feces of mice supplemented with this strain was significantly higher than the control group from day six as detected by pcr (). on day 14, qrt-pcr detection showed that the rate of jl-3 had reached 10−6(p<.001) (). further analysis also showed that compared with the ua group, the colonization of jl-3 reduced ua content in the blood and urine of mice. from day one to day three, ua levels of the ua group and jl-3 ua group both increased rapidly, reaching more than 15 times that of the control group (p<.0001). however, ua levels of the jl-3 ua group in urine gradually decreased from day three, and approached the control level on day nine. in serum samples collected on day 15, ua levels of the ua group (249.9µmol/l) were 2.2 times higher than the control (112.6µmol/l). after intervention with jl-3, ua concentrations decreased by about 31.3% compared with the ua group (p<.01) (). compared with the control group, there was no significant difference between ua levels of urine and blood samples from the jl-3 group, which demonstrated that jl-3 could effectively reduce ua in hyperuricemia mice ().
figure 3.effect of jl-3 on hyperuricemia mice. a]the hyperuricemia mouse model was established by detecting ua in urine and serum. in 2% concentration of ua and 4% of op group, the ua level was significantly high, which was selected for experiment on jl-3 treatment.b]experimental chart of jl-3 in treatment of hyperuricemia mice.c]detection of jl-3 colonization by pcr at 4, 6, 8, and 10 d.d]the proportion of jl-3 in fecal flora determined by rt-pcr on day 22.e]the content of ua in the urine after 0, 2, 4, 6, 8, 10, and 12 d.f]the serum ua of mice after the experiment. bars show the mean ± sd (n=5 mice per group) **p<.01; ****p<.0001
2.4. jl-3 strain attenuated ua-induced oxidative stress and inflammation
to further analyze the effect of jl-3 on the physiological indexes of mice, the levels of il-1β, malonaldehyde (mda), creatinine (cre) and blood urea nitrogen (bun) were tested, which all showed an increasing trend in the ua mice group (p<.01) (, b, c, d). also, when compared with the ua group, levels of these markers in the serum of the jl-3 ua group were greatly reduced (p<.01). specifically, il-1β was reduced by 50.9%, bun by 66.7% (accounting), mda by about 40%, and cre by 73%. in the kidney and liver, il-1β level in the ua group was significantly increasedp<.01). however, it partially dropped in the jl-3 ua group (, g). alternatively, xanthine oxidase (xod) levels were increased in the liver of the ua group and restored to the control level after jl-3 intervention (). also, there was no significant difference in the kidney mda level among the four groups (). histopathological analysis showed that ua exposure induced liver damage, as liver sections from the ua mice group showed that cell space was increased, and nuclear staining deepened compared with the control mice. however, these symptoms were partially relieved in the jl-3 ua group. however, they were no significant difference between the four groups in the kidney ().
figure 4.inflammation markers and oxidative stress indicators on hyperuricemia mice processed by jl-3. levels of interleukin-1β (il-1β) (a), mda (b), cre (c), bun (d) in mice serum in four groups.e, f]levels of interleukin-1β (il-1β) and mda in mice liver.g, h]levels of interleukin-1β (il-1β) and the content of xod in mice kidneys in different groups.i]representative photomicrographs of h&e staining of mouse liver tissue. mice were killed after feeding for 15 d, and serum was harvested from whole blood. bars show the mean ± sd (n=5 mice per group). **p<.01; ****p<.0001
figure 5.effects of jl-3 treatment on the function and microbial diversity of the gut microbiota in ua-exposed mice. a]degradation of ua by fecal flora of mice in different groups.b]the concentration of short-chain fatty acids in mouse feces.c]principle-coordinates analysis (pcoa) of the overall diversity based on the bray-curtis distances. scatter plot of pcoa scores depicting variances derived from bacterial communities in the four groups.d]comparison of phylum relative abundance in different groups.e]the relative abundance of the most abundant bacterial genera in the four groups.f, g, i]relative abundances of significantly changed bacterial phyla (bacteroidetes, firmicutes, andacidobacteria).h]the firm/bac ratio in the different groups.i]venn diagram analysis of otu overlaps between different groups of microorganisms. the compared model group, it can modulate the intestinal flora structure when mice were given jl-3. bars show the mean ± sd (n=5mice per group) **p<.01; ****p<.0001
2.5. jl-3 regulated ua-induced gut microbiota dysbiosis in hyperuricemic mice.
to investigate whether jl-3 could influence ua decomposition ability of the intestinal flora, we compared the ua degradation ability of feces bacteria in each group. as shown in, after 72h of cultivation in ua medium, the feces supplemented with jl-3 mice degraded more than 55% of the initial ua in the medium (p<.0001). scfas detection by gc-ms in the feces of mice on day 15th, revealed that the total scfas and acetic acid concentrations in feces of the ua group were significantly lower than the control (p<.01). they were recovered in the jl-3 ua group, but showed difference with the control group. in the jl-3 group mice feces, the butyric acid concentration also significantly increased compared with the control group (p<.01) ().
after that, we performed miseq sequencing analysis of 16s rrna to determine the diversity and abundance of gut microbiota associated with ua and/orlimosilactobacillus fermentum jl-3treated mice. at the end of the experiment, the abundance of microbial flora (shannon index) was higher in ua jl-3 group than the ua group, which was regulated by jl-3 treatment (supplementary table 1). alternatively, principle-coordinates analysis (pcoa) results showed that phylogenetic community structures were markedly different between ua-exposed samples and others, and that the ua group was separated from other groups. it suggested that ua exposure changed the gut microbiota composition, while jl-3 supplement diminished the effect of ua on microbiota changes (). the relative abundance at the phylum level of each individual sample in four groups was analyzed as shown in. the dominant phyla in the gut microbiota werebacteroidetes, firmicutes, proteobacteria, epsilonbacteraeotaandacidobacteria(relative abundance > 0.5%). comparing the ua mice group to the control, there was a significant increase in the relative abundance ofbacteroidetesand a reduction infirmicutesandproteobacteria. jl-3 treatment attenuated ua-induced increase inbacteroidetesand reduction infirmicutesandacidobacteria(, g, i). the ratio offirmicutestobacteroidetes(firm/bac ratio) was also deceased on ua exposure mitigated by jl-3 treatment (). at the genus level (), results showed that the relative abundance oferysipelatoclostridium and mucispirillumincreased after ua exposure, while that ofalloprevotellaandoscillibacterdecreased. jl-3 administrations countered ua-induced changes of the intestinal flora as well. in the jl-3 ua group,alloprevotellaandoscillibacterlevels were higher than the ua group by 107.15% and 125.22%, respectively. the abundance oferysipelatoclostridiumandmucispirillum, 64.01% and 65.65% respectively, were also less than that of the ua treated group, which had high levels in the diseased organism’s body.venn analysis also showed that ua treatment significantly affected the structure of the intestinal flora (). all these results indicated that jl-3 has a positive effect on gut microbiota.
3. discussion and conclusion
3.1. discussion
hyperuricemia continues to pose health problems around the world.although its prevalence is influenced by genetic factors, alcohol consumption, obesity, and hypertension are important factors that partially account for the recorded ua burden per time.gut remediation is a simple, economic, and effective measure for humans by introducing specific probiotics as dietary supplements.,thus, fermented foods rich in bacteria have been reported effective against many diseases.previous studies have authenticated the effects of fermented foods in effectively relieving diseases through their antioxidant activityin-vivo.,kimchi has shown an antioxidant effect in the prevention of atherosclerosis.cheese lowers the risk of cardiovascular diseases by 2%.meanwhile, previous research also proved that lactic acid bacteria in fermented foods have protective effects against some heavy metals and antibiotic residues in mammals.,scientific reports have also shown that jiangshui noodles are rich in lactic acid bacteria.furthermore, a survey reported that the incidence of gout in northern areas including gansu province was approximately 0.5% lower than the national average level (1.1%), which was coincident with our results.studies have also shown that jiangshui has a high degrading capacity of 75%.therefore, we proposed that the effect on gout is due to the action of probiotics present in fermented jiangshui.
this study focused on probiotic treatment for hyperuricemia, while earlier studies mainly concentrated on the purine metabolism, and only a few focus on ua.,,ua has been established as the major etiologic factor in hyperuricemia.research has also found that it can be used as a nitrogen source by many aerobic organisms, such asescherichia coli. the general catabolic pathway of ua by bacteria is allantoin, which proceeds via three steps, involving the interconversion between 5-hydroxyisouric acid, 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline and allantoin, which could easily degrade to ammonia.,in our study, allantoin was detected as a by-product of jl-3, which is coincident with former reports, as its degradation rate was found to be more significant compared withbacteroides termitidisuad-50 (50µmol/2h).,
after 15 d of intervention, the amount of jl-3 in the feces exceeded 106cfu/g, which was in accordance with a previous study, thus indicating that the jl-3 strain continued to survive in the mouse intestine throughout the experiment.the beneficial effect of jl-3 on hyperuricemia mice was evidenced by the decreased ua levels, as results showed that the ua levels in jl-3 ua group significantly decreased to more than 30% in feces and urine compared with the ua group. this was effectively reduced compared with dm9218 (< 30%) and the microbial fermented extracts oflactobacillus(21.6%).,consistent with previous studies, we found a dramatic increase in bun and cre after ua injection, which is associated with hyperuricemia, chronic nephritis and renal insufficiency.,in addition, serum mda was also increased, as was the il-1β in the liver and kidney, which is similar to the high-fructose diet.however, after jl-3 treatment, the levels of all these parameters were modulated to normal. xod catalyzes the hydroxylation of hypoxanthine and xanthine, which are the last two steps of urate biosynthesis.after intervention with jl-3, increased xod activity in the liver was restored to normal, suggesting that jl-3 inhibited xod. similar xod-inhibiting effects were reported in microbial fermented extracts ofaspergillusfungi (40.8% – 63.5%), but the underlying mechanism is unclear and deserves further investigation. this suggests that ua-induced oxidative stress and inflammatory response in the liver. the over production of xod has been demonstrated to cause an increased production of ua to further increase inflammation. in addition, consistent with previous research onl. acidophiluskb27 andl. rhamnosuskb7958, h&e photomicrographs further showed the characteristics of injury in hyperuricemia mice kidney that was significantly reduced after the jl-3 intervention.,
specifically, the ua degradation capacity of fecal bacteria in ua jl-3 mice was remarkably higher than that in mice treated solely with ua. this suggested that the ua-degradation ability of fecal bacteria in the ua jl-3group was enhanced by the addition of jl-3. the restorative effect of jl-3 on the metabolism of gut microbiota was also evidenced by the recovery of intestinal scfas. studies have shown that scfas are important energy and signaling molecules produced by intestinal microorganisms associated with gout. similar to the acetic acid in the intestine, scfas can serve as a central regulator for the intensity and duration of gout inflammation.,this revealed that jl-3 could restore intestinal metabolism and promote ua degradation.
gut microbiota is characterized by temporal stability and resilience.studies have demonstrated that the gut microbiota might be related to gut inflammatory responses, which participate in a series of diseases.however, many external factors that exceed the tolerance capacity of the gut microbiota can lead to dysbiosis.as an antioxidant and immune enhancer, ua exerts a great impact on the gut microbiota community.ua exposure significantly reduced the diversity and abundance of gut microbiota compared to those of the control group mice, which was regulated by jl-3 treatment, suggesting that jl-3 improves the gut microbiota disturbed by ua exposure. at the phylum level, ua significantly increased the relative abundance ofbacteroidetes, but decreased that offirmicutesandacidobacteria, which impart key functions to their host, such as metabolic, developmental, and immunologic properties.this is similar to a previous research based on the sprague–dawley rats with hyperuricemia.however, jl-3 pretreatment significantly attenuated ua induced increase inbacteroidetesand decrease infirmicutesandacidobacteria. at the genus level,prevotellaandbacteroidaleswere negatively associated with testicular function, whileparaprevotella, mucispirillum, oscillospira, coprococcusandlactobacilluswere positively correlated with that function.,jl-3 pretreatment reduced the increase ofprevotellaandbacteroidales, and elevated the decrease ofparaprevotella, mucispirillum, oscillospiraandlactobacillusin ua-exposed mice by regulating intestinal flora and improving intestinal function to alleviate gout.,in addition, the low abundance inherent strain oferysipelatoclostridiumthat appears in a host colitis by competing for nutrients negatively affected the flora.,in correspondence with our reports,l. acidophiluskb27 andl. rhamnosuskb79 supplements also confirmed the fact that mice gut microbiota were rich infirmicutesandbacteroidetes.thus, it can be concluded that the feed addition of jl-3 can reduce the phylum level disturbance associated with hyperuricemia and maintain the steady-state of the mice intestinal microbial community.
based on these experimental results, it is hypothesized that jl-3 might exert its effects in multiple ways. first, jl-3 can degrade ua in the gut, thus reducing the amount of ua that accumulates in the intestinal tract, thereby lessening the amount of ua reabsorbed by the intestinal epithelium reentering circulation. the protective mechanism of jl-3 against ua accumulation in mice was also found to be similar to several reports on purine-degradinglactobacillusstrains.,,second, jl-3 can improve bowel movement activity, thus decreasing fecal excretion of ua. thirdly, jl-3 can modulate the structure and function of gut microbiota.
3.2. conclusion
in this study,limosilactobacillus fermentumjl-3, a candidate probiotic strain was derived from fermented dairy products with ua reduction capability. this study explored the degradation of ua by jl-3 using mice as an animal model. the bacteria administered to mice, colonized the intestine, while jl-3 decreased ua levels in mice, and regulated the structure and function of the gut microbial community. compared to current treatment technologies, probiotics appear to be direct and efficient at repairing hyperuricemia, thus playing an important role in hyperuricemia treatment in humans, which might provide a promising strategy for alleviating hyperuricemia.
4. materials and methods
4.1. questionnaire on “jiangshui” noodle consumption
a cross-sectional comparison of the questionnaire with a standard chart abstraction-based measure (pearson index) was conducted on volunteers among the population in gansu province (supplementary file1). the questionnaire survey was conducted anonymously to count the gout conditions, and statistically investigate the incidence of related behaviors. the prevalence of gout was investigated in 180 residents with eight questions regarding gout. the survey mainly focused on: 1) the frequency of jiangshui noodle consumption every week; 2) the situation of gout; 3) the frequency of dietary habits such as drinking and eating meat. spss v.17.0 software was used to analyze the data atp< 0.05. the difference was statistically significant.
4.2. isolation of the bacterial strain with ua degrading ability
traditional jiangshui samples were collected from lanzhou supermarket (gansu, china). from the collected samples, 20 strains were isolated in a ua medium (1.71 g na2hpo4. 12 h2o, 0.3 g kh2po4, 0.05 g nacl, 0.05 g mgso4.7 h2o, 0.001 g cacl2, 0.2 g ua and 1.2 g agar, per 100ml) (supplementary table 2).the selected strains were then transferred to a new medium with ua as the sole carbon source. the grown bacterial culture plates were kept in an incubator at 37°c for 48 h. while the obtained bacterial colonies (jl-2, jl-3 and jl-8) were further streaked on new mrs agar plates to acquire a single colony. the new monoclonal colony activated in mrs medium was then stored in 25% glycerol at −80°c.
4.3. determination of uric acid degradation ability of strains in vitro
to evaluate the ua degradation ability, jl-2, jl-3 and jl-8 were inoculated onto mrs and cultured for 48 h at 37°c under anaerobic conditions. 2 ml of the culture solution was centrifuged at 4,000 ×gfor 10 min, after which the cells were washed twice with 1 ml 0.85% nacl, resuspended in 750 ml of ua solution and incubated at 37°c for 60 min. later, the solution was centrifuged at 4,000 ×gfor 10 min to obtain the supernatant. thirty milliliters hclo4(0.1 mol/l) was then added to the supernatant and mixed thoroughly to prevent further degradation, after which 20 ml of the mixture was injected into the hplc device after filtration. the remaining urea and allantoin contents were calculated. the degradation rate of jl-3 strains was also calculated according to the following formula: v = (0.9 c − x)/60, a = [(0.9 c − x)/0.9 c] n100%.
where v: degrading speed (g/l/min), x: the remaining content of urea or allantoin (g/l).,adenylate-guanylate-neutral potassium phosphate culture solution (900 μl) was mixed with 100 μl of terminator perchloric acid solution. after mixing, 20 μl was aspirated. the external standard method was then used to determine the retention time of ua to generate a quantitative standard curve. the conditions for hplc were as follows: water xterra ms c18 (250 mm × 4.6 μm), the mobile phase was a gradient of 20 mmol/l potassium dihydrogen phosphate solution (ph 5.0), with a flow rate of 1 ml/min, while column temperature was 25°c, with wavelengths of 254 nm (ua), 190 nm (urea), 190 nm (allantoin), and an elution time of 40 min.
4.4. 16s rrna gene sequencing and phylogenetic analysis
tianamp bacteria dna kit (tiangen biotechnology, china) was used to obtain high quality genomic dna of strains. the 16s rdna was amplified as described by sibleyet al.the sequence of potential isolates for species identification was made using the blast engine (ncbi) and the nucleotide sequence was submitted to genbank for reference (sub8885938 seq1 mw474833, sub8885938 seq2 mw474834, sub8885938 seq3 mw474835).the morphology of jl-3 was analyzed by scanning electron microscopy (sem s-3400n ii hitachi, japan), while the multiple sequences of jl-3 and other closely related strains were aligned by mega (molecular evolutionary genetics analysis, v.6.0).,
4.5. experimental design based on the animal model
fifty kunming male mice (lanzhou university medical animal testing center) which were 4-week-old and weighed 16–20 g, were bought. before the experiment, mice were habituated for a week. for the experimental procedures, 1 mouse per cage was housed at 22°c ± 1°c and 60%–75% relative humidity. the mice were then fed with standard commercial mouse food. the care and use of laboratory animals in our study was approved by the lanzhou university animal ethics committee, after that the relevant ethical regulations were followed.
for inducting of ua-induced hyperuricemia, mice were given oteracil potassium (op) and ua in 0.5 ml water for 14 d. fifty mice were randomly divided into five groups, where op and ua were used to construct the hyperuricemia animal model according to literature.,the control group was then fed with a basal diet, and orally gavaged with 0.5 ml of water, while the ua1group was given the basal diet along with op (4%) and ua (1%) injection through the oral gavaged route. the ua2group was also given the basal diet of op (4 %) and ua (2 %), which were injected through the oral gavaged route, while the ua3group was fed the basal diet and intraperitoneally injected with op (4%) and ua (2%) in 0.5 ml water. the blank group was then fed with a basal diet and intraperitoneal injection of 0.5 ml water. during this period, urine and blood samples were collected to determine whether the model was well established by detecting the ua content. according to an earlier research, different concentrations of ua were selected to feed mice by injection to establish hyperuricemia model mice.
the 2% concentration of ua and 4% of op confirmed during the model experiment were used for repair experiments. forty mice were randomly allocated into four groups (10 mice per group). the control group was fed with the basal diet and sterile skim milk (0.5 ml) administrated by oral gavage every day, while the jl-3 group was also fed with a basal diet and gavage with jl-3 in the sterile skim milk. the ua group, on the other hand, was fed with basal diet plus 2% ua and 4% op, and orally gavaged sterile skim milk (0.5 ml), while the ua jl-3 group received a basal diet containing 2% ua and 4% op, while being orally gavaged with the jl-3 strain in sterile skim milk. about 1×108cfu of jl-3 was dissolved into 0.5 ml of skim milk, which was directly injected into the mouth of each mouse twice a day (9:00 in the morning and 18:00 in the afternoon). skim milk was prepared using sterilizing skimmed milk powder at 15 min, while 1 ml of bacterial solution was centrifuged at 8,000 ×gfor 10 min, after which the suspension of the sterilized milk was used.
during the 15 d of treatment, all mice in each group were moved into a clean and empty cage every three days for an hour, and fecal and urine samples were collected. later, the mice were anesthetized using diethyl ether. blood was collected from the eye vein and was left undisturbed for 30 min. the collected blood samples were centrifuged at 4000 rpm for 20 min to get the serum. also, liver, kidney and intestine samples were gathered for the respective groups. the collected samples were then washed with saline, collected in pre-weighted tubes with 1 ml pbs diluent, and stored at −20°c for future use. all samples were preserved at −80°c until analysis, except those used for histopathology, which were stored in 4% paraformaldehyde.
4.6.colonization oflimosilactobacillus fermentumjl-3
during the experiment, mice feces were collected after 4, 6, 8, and 10 d. pcr was then performed using specific primers of thelimosilactobacillus fermentumas reported in previous literatures.,all primer sequences for qpcr are listed: specific primers of jl-3 (f: acgtatgaacagttactctcatacgt; r: cctgattgatttggtcgccaac; and general primers of bacteria (f-tot: gcaggcctaacacatgcaagtc; r-tot:ctgctgcctcccgtaggagt), were used to obtain pcr products. then, semi-quantitative fluorescence analysis of jl-3 was performed using image j-win v.32.
on the 22nd day, fresh mouse feces were cultured in the mrs medium, and incubated for 48 h at 37°c in a static incubator, then sub-cultured again. mouse fecal dna was then extracted according to the manufacturer’s instructions using tianamp stool dna kit. to quantify the total bacteria in the feces samples, primers tot-f and tot-r were used for amplifying the 16s rdna, while was run on a bio-rad cfx96 (usa) in a total volume of 10 μl, using sybr premix ex taq ii (takara) on real-time quantification pcr instrument (bio-rad cfx96, usa). all measurements were performed in triplicate. standard curves were then constructed using pcr products of the 16s rrna gene of jl-3 ande. coli, after which the obtained pcr products were cloned into t vector (takara, dalian, china) and transfected into dh-5α, which was then incubated at 37°c for 6 h (100 −140 rpm) by plasmid mini kit i (e.z.n.a., omega, usa) as the standard sample. the recombinant plasmids were used for qpcr, with 103, 104, 105, 106, 107, 108, 109, and 1010copies of the plasmids per reaction used for calibration. the target copy numbers (t) were then estimated using the equation: t = (d/(pl × 660)) × 6.022 × 1,023,133, where d (g/l) and pl (base pairs) were the plasmid dna concentrations and lengths, respectively. each standard curve was then generated from at least five 10-fold plasmid dilutions in triplicate. the proportion of jl-3 in the whole bacterial community of bacteria in different samples was calculated based on the standard curve.,
4.7. determination of ua in mice
the frozen mouse feces in 1 ml pbs were placed in a 60°c water bath for 10 min, and vortexed for 1 min to break up the stool sample. the solution was collected by centrifugation at 1000 rpm for 5 min. fresh mouse urine was placed in 60°c water bath and diluted 10 times with distilled water to ensure that no urate deposits were present in the urine sample, after which all samples were strictly detected according to the instructions provided in the ua detection kit (jianchengnanjing, china). this kit provides a fluorescence-based method for detecting ua. in the assay, uricase transforms ua to allantoin, hydrogen peroxide (h2o2), and carbon dioxide. in the presence of horseradish peroxidase, h2o2reacts with 10- acetyl-3, 7-dihydroxyphenoxazine to produce the highly fluorescent compound resorufin. resorufin fluorescence was then analyzed at an excitation wavelength of 530 nm and an emission wavelength of 595 nm, after which the concentration of ua in the serum and urine samples were calculated using the equation determined from different doses of standards.
4.8. determination of short-chain fatty acid (scfa) in mouse feces
the fecal samples were frozen immediately after collection and stored at −20°c until analysis, while 1g of the feces sample was thawed and suspended in at least 5 ml water and homogenized for about 3 min, resulting in a 17% (w/w) fecal suspension. the ph of the suspension was then adjusted to 2–3 by adding 5 m hcl, and then kept at room temperature for 10 min under occasional shaking. the suspension was then transferred into a polypropylene tube and centrifuged for 20 min at 5000 rpm, to give a clear supernatant, while the internal standard, 2-ethylbutyric acid solution (2eb), was spiked into the supernatant at a final concentration of 1 mm and injected in the gc for analysis. the response linearity for fecal samples was tested with the serial solutions prepared by diluting the above serial standard solutions with equal volume of fecal supernatant, after which the concentration of scfas were measured by gc-ms chromatographic workstation. n2 was used as the carrier. the concentration of scfas in the supernatant was detected by chromatographic column (ffap 30 m × 0.32 mm × 0.5 µm) and fid detector, and the properties of scfas were determined afterward. the content of scfas in each sample was then calculated by comparing with the internal standard 2eb.
4.9. tissues collection and biomarker measurements
fresh liver and kidneys were ground using a handheld grinder (jingxin, shanghai, china) on ice, after which the suspension of samples was obtained at low speeds centrifugation (8000 rpm, 10 min). the levels of xod and il-1β in the liver, mda and il-1β in kidney, and il-1β, mda, creatinine (cre), and urea nitrogen (bun) in serum samples were all then detected using commercial kits (jiancheng, nanjing, china) according to the protocols provided. at least 5 samples from each group were selected for independent assay.
the liver and kidney samples were then rinsed with pbs, after which the liver was taken with a volume of 0.8 × 0.8 cm, while whole kidneys were employed. the samples were fixed with 4 % paraformaldehyde solution at room temperature for more than one day. later, the samples were sent to the medical college of lanzhou university for physiological sections and staining with hematoxylin and eosin (he). the morphology of the sections and the accumulation of ua in mice renal tubules in different groups were observed under a microscope (olympus bx53, japan) afterward.
4.10. degradation ability of ua by mice fecal flora
mice stool samples were collected at different time points. at first, 1 g of stool was cultured in the ycfa medium as reported in literature,and then incubated for 48 h at 37°c in an anaerobic incubator. the samples were then transferred to a new anaerobic incubator for two days. the cell content in the medium was detected using od600(metash uv-5100). the media contents were centrifuged at 8,000 × g for 10 min with an equivalent community to obtain cell pellets (handheld tissue grinder, jingxin my-10, china). the obtained cell pellets were then washed twice with pure sterile pbs and resuspended in pure pbs solution containing ua. supernatant of the solution was taken from the anaerobic incubator every three days. the ua detection kit (jianchengnanjing, china) was used to detect the ua concentration changes according to the direction of the manufacturer.
4.11. dna extraction and 16s rrna amplification
the feces were collected from individually housed mice the day before gavage treatment initiation, and stored at −80°c until dna collection. the stool sample pellets were weighed to 0.2 g and dna, isolated in ddh2o using a modified tianamp stool dna kit (jianchengnanjing, china) protocol. the obtained dna was then stored at −20°c until use. according to the selection of sequencing regions, diluted genomic dna served as templates, with specific primers having barcode and high-fidelity enzymes (takara, dalian) to ensure pcr amplification efficiency and accuracy. pcr was performed for 16s v4-v5 region of bacteria using a primer pair 515f (5′-gtgycagcmgccgcggtaa-3′) and 806r (5′-ggactacnvgggtwtctaa-3′).the pcr mixture (25 μl) contained 1x pcr buffer, 1.5 mm mgcl2, and deoxynucleoside triphosphate at 0.4 μm, each primer at 1.0 μm and 0.5 u of ex taq (takara, dalian) and 10 ng soil genomic dna. the pcr amplification protocol included initial denaturation at 94 °c for 3 min, followed by 30 cycles of 94°c for 40 s, 56°c for 60 s, and 72°c for 60 s, and a final extension at 72°c for 10 min. two pcr reactions were conducted for each sample, and combined together after pcr amplification.dna concentration and purity were measured by nanodrop (thermo scientific, usa). the pcr product was detected by 1% agarose gel electrophoresis. the product was recovered from the target strip using the dna gel extraction kit (omega), and the concentration and quality of the product were determined by nanodrop. the same amounts of samples were mixed according to the concentration of pcr products. the library was constructed with truseq® dna pcr-free sample preparation kit and quantified using qubit and qpcr (prjna691734). later, it was sequenced by an illumina sequencing platform (miseq pe250),and the resulting sequence read files using the qiime2 pipeline (quantitative insights into microbial ecology;) and its plugins. paired-end sequence splicing was also performed using flash (v.1.2.7,).,the reads of each sample were spliced, and the spliced sequences were raw tags. singleton was removed from otus (otus with only one sequence in all samples). the otu representative sequences were annotated by qiime2 software, using silva-132-99-515-909-nb-classifier.qza as a reference database. alternatively, the chloroplast and mitochondrial sequences of eukaryotes were removed, and qiime2 software used to analyze observed species, evenness, shannon, faith’s pd and other diversity indexes were calculated, and dilution curve was drawn afterward. according to otu table and phylogenetic tree, unifrac distance matrix was generated and weighted, after which the unifrac pcoa map was drawn.
4.12. statistical analysis
statistical analysis was performed using spss v.17.0 software.p-value < 0.05 indicated statistical significance. data were analyzed using one-way analysis of variance (anova). the significant differences between the two groups were analyzed using an independent samples t-test. to determine the composition of the bacterial community, pcoa was performed using rversion v.3.3.2.
acknowledgments
the work was supported by gansu province major science and technology project (grant no: 17zd2wa017), national natural science foundation of china (grant no: 31870082), fundamental research funds for the central universities (grant no: lzujbky-2020-83) and cheng guan district science and technology development project (grant no: 2020jscx0019). we thank the central lab of college of life science, lanzhou university for providing all necessary equipment.
disclosure statement
the authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. author declare no conflict of interest.
credit authorship contribution statement
ying wu: conceptualization, investigation, data curation, writing - original draft.ze ye: conceptualization, moleculer biology, data curation and investigation, writing - review & editin.pengya feng: writing - review & editing.rong li and xiao chen: validation and formal analysis.xiaozhu tian: supervision.rong han: writing - review & editing.apurva kakade: writing - review & editing.pu liuandxiangkai li: supervision and funding acquisition.
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additional information
funding
this work was supported by the fundamental research funds for the central universities [grant no: lzujbky-2020-83]; national natural science foundation of china [grant no: 31870082]; gansu province major science and technology project [grant no: 17zd2wa017]; chengguan district science and technology development project [grant no: 2020jscx0019].